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KMID : 0895820030130040063
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Journal of Oriental Rehabilitation Medicine
2003 Volume.13 No. 4 p.63 ~ p.89
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Polymorphism of β2-adrenergic receptor, β3-adrenergic receptor, ACE, G protein β3 and MTHFR gene in Korean Obese Female Subjects
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Lee Sang-Ho
Shin Hyun-Dae
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Abstract
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Objectives: There have been several reports on the relationship between β2-adrenergic receptor(B2AR), β3-adrenergic receptor(B3AR), G protein β3(GNB3), Angiotensin converting enzyme(ACE), Methylenetetrahydrofolate reductase(MTFHR) genotype and obesity or obesity related disease. The objective of this study was to examine the relationship between these polymorphism and obesity in Korean female subjects.
Methods: The study group was consisted of 85 Korean obese female subjects(Body Mass Index; BMI ≥ 27 kg/m2) and 80 normal-weighted Korean subjects(BMI < 25 kg/m2). To determine the polymorphism, genomic DNA was isolated, and polymerase chain reaction was carried. Serum lipid and blood glucose were measured by an autoanalyzer. BMI, body fat distribution ratio, waist circumference, hip circumference and waist hip ratio were measured.
Result:
(1) Frequencies of B2AR genotype(codon 16) in obesity group were Gly16 homozygote type 37.6%, Gly16/Arg16 heterozygote type 55.3%, Arg16 homozygote type 7.1%, in control group were, respectively, 38.8%, 43.8%, 17.5%. Frequencies of the genotype were not significantly different between obesity and control group. Gly16 homozygote type subjects had an increased risk of obesity(OR = 3.132, 95% CI 1.094 - 8.695, P = 0.033).
(2) Frequencies of B3AR genotype in obesity group were Trp64 homozygote type 67.1%, Trp64/Arg64 heterozygote type 32.9%, Arg64 homozygote type 0.0%, in control group were, respectively, 72.5%, 26.3%, 1.3%. Frequencies of genotype were not significantly different between obesity and control group.
(3) Frequencies of GNB3 genotype in obesity group were C/C type 14.1%, T/C type 55.3%, T/T type 30.6%, in control group were, respectively, 30.0%, 45.0%, 25.0%. Frequency of genotype were significantly different between obesity and control group(Chi-square test, Pearson Chi-Square value: 6.095 P = 0.047). Frequency of the T allele, also, was significantly different between obesity and control group(Chi-square test, Pearson Chi-Square value: 6.094, P = 0.014). T allele subjects had an increased risk of obesity(OR = 2.607, 95% CI 1.200 - 5.661, P = 0.015).
(4) Frequencies of ACE genotype in obesity group were D/D type 14.1%, I/D type 40.0%, I/I type 45.9%, in control group were, respectively, 17.5%, 45.0%, 37.5%. Frequencies of the genotype were not significantly different between obesity and control group.
(5) Frequencies of MTHFR genotype in obesity group were C/C type 20.0%, T/C type 71.8%, T/T type 8.2%, in control group were, respectively 28.8%, 57.5%, 13.8%. Frequencies of the genotype were not significantly different between obesity and control group.
Conclusions: These results suggest that the Gly16 homozygote type in B2AR gene and T allele in GNB3 gene appear to be a genetic risk factor for Korean obese female subjects.
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KEYWORD
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β2-adrenergic receptor gene, β3-adrenergic receptor gene, Angiotensin converting enzyme gene, G protein β3 gene, Methylenetetrahydrofolate reductase gene, Polymorphism, Obesity
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